Klaus Bahl Andersen

Master degree in biochemistry/microbiology for a work on bacterial respiration and nucleotide metabolism. Earned a Ph. D. for studies on eukaryotic translational initiation complexes, followed by fellowships at The Danish Cancer Society working on retrovirus.

 

Curriculum Vitae

Born  Oct. 12. 1949.

M.Sc. (Cand. scient.) in biochemistry/microbiology at University of Copenhagen, 1975.

1976-1978 Post. Doc. At University of Pittsburgh, Department of Biological Sciences.

Ph D. (Lic. Scient.) from University of Copenhagen,1978.

1978-1982 Danish Cancer Society, fellowship.

1983- Associate Professor at Danish University of Pharmaceutical Sciences.

 

Research interests

Present research concerns the surface proteins of retroviruses both in respect to the infection process and the assembly process.

Recently the interest has been on the murine leukemia virus transmembrane R-peptide, to which we have developed an antiserum. R-peptide is located on the C-terminal, cytoplasmic tail of the transmembrane protein, which is cleaved off, rendering the transmembrane protein fusinogenic. We found that the R-peptide in the cells is attached to palmitic acid (Olsen, Andersen, 99) though it has not so far been possibly to link the palmitic acid to a specific amino acid (Zedeler, thesis, 2005)

 and most recent that the R-peptide and also uncleaved Transmembrane protein is bound small core like complexes (1,23 g/ml 150-200 s Gag containing complexes) which possibly is preliminary stage of virus particle development (Andersen, Diep, Zedeler, 06).      

 

 

List of publications

Andersen KB, Diep HA, Zedeler A  Murine leukaemia virus transmembrane protein R-peptide is forum in small virus core-like complexes in cells. J Gen Virol. 2006: In Press, expected in June issue.

Smith L, Andersen KB, Hovgaard L, Jaroszewski JW.  Rational selection of antisense oligonucleotide sequences.  Eur J Pharm Sci. 2000 Sep;11(3):191-8.

Olsen KE, Andersen KB.  Palmitoylation of the intracytoplasmic R peptide of the transmembrane envelope protein in Moloney murine leukemia virus.
J Virol. 1999 Nov;73(11):8975-81.

Andersen KB, Olsen KE.  Fusion between uninfected cells in retrovirus-induced fusion-from-within.  Virus Res. 1998 Nov;58(1-2):53-64.

Andersen KB.  K+ ionophores stimulate retrovirus-induced fusion-from-within.
Virus Res. 1998 Apr;54(2):157-64.

Andersen KB.  Characterization of retrovirus-induced SC-1 cell fusion.
Virus Res. 1995 Aug;37(3):177-98.

Andersen KB.  A domain of murine retrovirus surface protein gp70 mediates cell fusion, as shown in a novel SC-1 cell fusion system.
J Virol. 1994 May;68(5):3175-82.

Skov H, Andersen KB.  Mutational analysis of Moloney murine leukaemia virus surface protein gp70.  J Gen Virol. 1993 Apr;74 ( Pt 4):707-14.

Andersen KB, Skov H.  Retrovirus-induced cell fusion is enhanced by protease treatment.
J Gen Virol. 1989 Jul;70 ( Pt 7):1921-7.

Andersen KB.  Cleavage fragments of the retrovirus surface protein gp70 during virus entry.  J Gen Virol. 1987 Aug;68 ( Pt 8):2193-202.

Andersen KB.  Leupeptin inhibits retrovirus infection in mouse fibroblasts.
J Virol. 1983 Dec;48(3):765-9.

Andersen KB, Nexo BA.  Entry of murine retrovirus into mouse fibroblasts.
Virology. 1983 Feb;125(1):85-98.

Andersen KB, Vaughan MH  Gel centrifugation  chromatrogrphy for macromolecular separations.  J Chromatog. 1982 May 7. 200:1-8

Andersen KB.  Association of moloney murine leukaemia virus proteins: an assay for hydrophobic protein-protein interactions.  J Gen Virol. 1982 Jan;58 Pt 1:83-93.

Andersen KB, von Meyenburg K.  Are growth rates of Escherichia coli in batch cultures limited by respiration?  J Bacteriol. 1980 Oct;144(1):114-23.

Andersen KB.  Methionyl-tRNA-Met-f deacylase. Purification, characterization, and effects on translational initiation complexes.  Eur J Biochem. 1979 May 2;96(1):109-18

Andersen KB, Bolesfoldi G, Vaughan MH.  Destabilization of eukaryotic 40 S translational initiation complex by ApUpG and partial characterization of a factor required for this activity.  J Biol Chem. 1978 Sep 25;253(18):6370-8.

Andersen KB, von Meyenburg K.  Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli.
J Biol Chem. 1977 Jun 25;252(12):4151-6.

 

US PATENT 4 422 941:

Vaughan MH, Andersen.  Apparathus for liquid-solid centrifugation chromatography and method

 

Teaching

Microbiology (B24) and Methods in Molecular Biology (b374)

Has developed teaching material for B24 (in Danish) of the topics: Bacterial growth,  The mode of action of disinfectants, Graph-drawing of results, Summary of viral pathogenesis,  Antiviral drugs.

Has further written sections in Lab manuals of B24 and B374. 

 

Supervision

Ph.D.'s

Helle Skov (1993): Infektionsvejen for retrovirus undersøgt ved in vitro mutagenese

Lise Moesby (1996): Viral membrane fusion. Interaction of Moloney murine leukaemia virus and Semliki virus with cellular and model membranes

Katharina E.P Olsen (1999): Analysis of the intracytoplasmic R-peptide of the transmembrane envelope protein in Moloney murine leukaemia virus.

Anne Zedeler (2005): Palmitoylation and raft localisation of the retrovirus Moloney MLV R-peptide studied by mutagenesis