Curriculum vitae for Arne Schousboe
Born June 9, 1944
Education:
June 1968: M.Sc. (biochemistry), Univ. of Copenhagen
May 1978: D.Sc., Univ. of Copenhagen
Employment:
1.9.68-1.6.73: Assistant professor, Dept. of Biochemistry A, Univ. of Copenhagen
1.6.73-31.12.89: Assoc. professor, Dept. of Biochemistry A., Univ. of Copenhagen
1.1.72-1.9.73: Research Fellow, Div. of Neurosci. (Dr. E. Roberts), City of Hope Natl. Med. Center, L.A. (USA)
1.1.90- : Professor, The Danish University of Pharmaceutical Sciences (Dept. of Pharmacology)
International reviewing activity:
Frequent reviewer for the following granting agencies:
Medical Research Council (UK and Norway)
National Science Foundation (USA)
International Human Frontier Science Program Organization (France)
The Wellcome Trust (UK)
Member of "BIOMED (EU) Impact Assessment Board 2001"
Member of the Danish Medical Research Council (1994-1999)
Membership of national and international societies:
Danish Academy of Natural Sciences (DNA)
Danish Society for Neuroscience
New York Academy of Sciences
European Society for Neurochemistry (Executive Council 1984-1990)
American Society for Neurochemistry
International Society for Neurochemistry (Executive Council 1995-1999; Secretary 1999-2003; President 2003-2005)
International Society for Developmental Neuroscience (Publications Committee; President 1994-96)
Elected member of Polish Academy of Science and Letters
Scientific publications and editing of books:
More than 450 articles in refereed journals and books. Editor of 7 books.
Registered in "Highly Cited Researchers" by ISI-Thomson Scientific (http://www.isihighlycited.com)
Research profile:
The research expertise includes studies of metabolism, transport and receptor functions for glutamate and GABA using a variety of methods including culturing of neurons and astrocytes. Receptor functions are also studied in a variety of expression systems such as Xenopus oocytes.
Recent publications (2001-):
Elster L., Kristiansen U., Pickering D.S., Olsen R.W. and Schousboe, A. Molecular determinants of desensitization and assembly of the chimeric GABAA receptor subunits ("1/(2) and ((2/"1) in combinations with $2 and (2. Neurochem. Int. 38, 581-592 (2001). www.neuroscion.com
Waagepetersen, H.S., Sonnewald, U., Gegelashvili, G., Larsson, O.M. and Schousboe, A. Metabolic distinction between vesicular and cytosolic GABA in cultured GABAergic neurons using 13C MRS. J. Neurosci. Res. 63, 347-355 (2001).
Timmermann, D.B., Lund, T.M., Belhage, B. and Schousboe, A. Localization and function of voltage dependent calcium channels in cultured neocortical neurons. Int. J. Devl. Neurosci. 19, 1-10 (2001).
Banke, T.G., Greenwood, J., Christensen, J.K., Liljefors, T., Schousboe, A. and Pickering, D.S. Identification of amino acid residues in GluR1 responsible for ligand binding and desensitization. J. Neurosci. 21, 3052-3062 (2001).
Waagepetersen, H.S., Shimamoto, K. and Schousboe, A. Comparison of effects of DL-threo-beta-benzyloxyaspartate (DL-TBOA) and L-trans-pyrrolidine-2,4-dicarboxylate (t-2,4-PDC) on uptake and release of [3H]D-aspartate in astrocytes and glutamatergic neurons. Neurochem. Res. 26 661-666 (2001).
Jensen, J.B., Lund, T.M., Timmermann, D.B., Schousboe, A. and Pickering, D.S. Role of GluR2 expression in AMPA-induced toxicity in cultured murine cerebral cortical neurons. J. Neurosci. Res. 65 267-277 (2001).
Waagepetersen, H.S., Sonnewald, U., Larsson, O.M. and Schousboe, A. Multiple compartments with different metabolic characteristics are involved in biosynthesis of intracellular and released glutamine and citrate in astrocytes. Glia 35, 246-252 (2001).
Schousboe, A., Hansen, G.H., and Carlson, B.X. Amino acid neurotransmitters as developmental signals. In: Brain and Behaviour in Human Development (Kalverboer, A.F. and Gramsbergen, A., eds.). Kluwer Academic Publ., The Netherlands, pp. 185-197 (2001).
Waagepetersen, H.S., Qu, H., Schousboe, A. and Sonnewald, U. Elucidation of the quantitative significance of pyruvate carboxylation in cultured cerebellar neurons and astrocytes. J. Neurosci. Res. 66, 763-770 (2001).
Zaganas, I., Waagepetersen, H.S., Georgopoupolos, P., Sonnewald, U., Plaitakis, A. and Schousboe, A. Differential expression of glutamate dehydrogenase in cultured neurons and astrocytes from mouse cerebellum and cerebral cortex. J. Neurosci. Res. 66, 909-913 (2001).
Campiani, G., Morelli, E., Nacci, V., Fattorusso, C., Ramunno, A., Novellino, E., Greenwood, J., Liljefors, T., Griffiths, R., Sinclair, C., Reavy, H., Kristensen, A.S., Pickering, D.S., Schousboe, A., Cagnotto, A., Fumagalli, E. and Mennini, T. Characterization of the 1H-cyclopentapyrimidine-2,4(1H,3H)-dione derivative (S)-CPW399, as a novel, potent and subtype-selective AMPA receptor full agonist with partial desensitization properties. J. Med. Chem. 44, 4501-4504 (2001).
Schousboe, A. and Kanner, B. GABA transporters: functional and pharmacological properties. In: Glutamate and GABA Receptors and Transporters (J. Egebjerg, A. Schousboe and P. Krogsgaard-Larsen, eds.). Taylor & Francis Publ., London, UK, pp. 337-349 (2002).
Schousboe, A. and Waagepetersen, H.S. Glial cell biology. In: The Newborn Brain - Neuroscience and Clinical Applications (H. Lagercrantz, M. Hanson, P. Evrard and C. Roedeck, eds.). Cambridge University Press, Cambridge U.K., pp. 166-176 (2002).
Timmermann, D.B., Westenbrook, R.E., Schousboe, A. and Catterall, W.A. Distribution of high voltage-activated calcium channels in cultured GABAergic neurons from mouse cerebral cortex. J. Neurosci. Res. 67, 48-61 (2002).
Barabás, P., Kovács, I., Kovács, R., Pálhalmi, J., Kardos, J. and Schousboe, A. Light-induced changes in glutamate release from isolated rat retina is regulated by cyclic guanosine monophosphate. J. Neurosci. Res. 67, 149-155 (2002).
Engblom, A.C., Carlson, B.X., Olsen, R.W., Schousboe, A. and Kristiansen, U. Point mutation in the first transmembrane region of the $2 subunit of the gamma_aminobutyric acid type A receptor alters desensitization kinetics of gamma_aminobutyric acid_ and anesthetic_induced channel gating. J. Biol. Chem. 277, 17438-17447 (2002).
Waagepetersen, H.S., Qu, H., Hertz, L., Sonnewald, U. and Schousboe, A. Demonstration of pyruvate recycling in primary cultures of neocortical astrocytes but not in neurons. Neurochem. Res. 27, 1431-1437 (2002).
White, H.S., Sarup, A., Bolvig, T., Kristensen, A.S., Petersen, G., Nelson, N., Pickering, D.S., Larsson, O.M., Frølund, B., Krogsgaard-Larsen, P. and Schousboe, A. Correlation between anticonvulsant activity and inhibitory action on glial GABA uptake of the highly selective mouse GAT1 inhibitor 3-hydroxy-4-amino-4,5,6,7-tetrahydro-1,2-benzisoxazole (exo-THPO) and its N-alkylated analogs. J. Pharmacol. Exp. Therap. 302, 636-644 (2002).
Jensen, M.L., Timmermann, D.B., Johansen, T.H., Schousboe, A., Varming, T. and Ahring, P. The $ subunit determines the ion selectivity of the GABAA receptor. J. Biol. Chem. 277, 41438-41447 (2002).
Schousboe, A. Role of astrocytes in the maintenance and modulation of glutamatergic and GABAergic neurotransmission. Neurochem. Res. 28, 347-352 (2003).
Sinclair, C., Reavy, H., Grieve, A., Schousboe, A., Morelli, E., Novellino, E., Campiani, G., and Griffiths, R. Inherent desensitization-preventing properties of a novel, subtype-selective AMPA-receptor agonist, (S)-CPW-399, as a possible explanation for its excitotoxic action in cultured cerebellar granule cells. Neurochem. Int. 42, 499-510 (2003).
Schousboe, A., Sonnewald, U. and Waagepetersen, H.S. Differential roles of alanine in GABAergic and glutamatergic neurons. Neurochem. Int. 43, 311-315 (2003).
Bak, L.K., Schousboe, A. and Waagepetersen, H.S. Characterization of depolarization-coupled release of glutamate from cultured mouse cerebellar granule cells using DL-threo-beta-benzyloxyaspartate (DL-TBOA) to distinguish between the vesicular and cytoplasmic pools. Neurochem. Int. 43, 417-424 (2003).
Bonde, C., Sarup, A., Schousboe, A., Gegelashvili, G., Zimmer, J. and Noraberg, J. Neurotoxic and neueroprotective effects of the glutamate transporter inhibitor DL-threo-beta-benzyloxyaspartate (DL-TBOA) during physiological and ischemia-like conditions. Neurochem. Int. 43, 371-380 (2003).
Bonde, C., Sarup, A., Schousboe, A., Gegelashvili, G., Noraberg, J. and Zimmer, J. GDNF pre-treatment aggravates neuronal cell loss in oxygen-glucose deprived hippocampal slice cultures: A possible effect of glutamate transporter up-regulation. Neurochem. Int. 43, 381-388 (2003).
Sarup, A., Larsson, O.M., Bolvig, T., Frølund, B., Krogsgaard-Larsen, P. and Schousboe, A. Effects of 3-hydroxy-4-amino-4,5,6,7-tetrahydro-1,2-benzisoxazol (exo-THPO) and its N-substituted analogs on GABA transport in cultured neurons and astrocytes and by the four cloned mouse GABA transporters. Neurochem. Int. 43, 445-451 (2003).
Rodriguez-Kern, A., Gegelashvili, M., Schousboe, A., Zhang, J., Sung, L. and Gegelashvili, G. Beta-amyloid and brain-derived neurotrophic factor, BDNF, up-regulate the expression of glutamate transporter GLT-1/EAAT2 via different signaling pathways utilizing transcription factor NF-6B. Neurochem. Int. 43, 363-370 (2003).
Bjerrum, E.J., Kristensen, A.S., Pickering, D.S., Greenwood, J.R., Nielsen, B., Liljefors, T., Schousboe, A., Bräuner-Osborne, H. and Madsen, U. Design, synthesis and pharmacology of a highly subtype selective GluR1/2 agonist, (RS)-2-amino-3-(4-chloro-3-hydroxy-5-isoxazolyl)propionic acid (Cl-HIBO). J. Med. Chem. 46, 2246-2249 (2003).
Sarup, A., Larsson, O.M. and Schousboe, A. GABA transporters and GABA-transaminase as drug targets. Curr. Drug Targ. CNS Neurol. Dis. 2, 269-277 (2003).
Frandsen, Aa. and Schousboe, A. AMPA receptor mediated neurotoxicity: Role of Ca2+ and desensitization. Neurochem. Res. 28, 1495-1490 (2003).
Barabás, P., Kovács, I., Kardos, J. and Schousboe, A. Exogenous glutamate and taurine exert differential actions on light-induced release of the two endogenous amino acids in the isolated rat retina. J. Neurosci. Res. 73, 731-736 (2003).
Waagepetersen, H.S., Sonnewald, U. and Schousboe, A. Compartmentation of glutamine, glutamate and GABA metabolism in neurons and astrocytes: Functional implications. Neuroscientist 9, 398-403 (2003).
Schousboe, A. and Waagepetersen, H.S. GABA. In: Encyclopedia of Neuroscience (G. Adelman and B.H. Smith, eds.). Elsevier Science, New York, USA, pp. 166-176 (2003) <
Schousboe, A. and Waagepetersen, H.S. Role of astrocytes in homeostasis of glutamate and GABA during physiological and pathophysiological conditions. In: Non-Neuronal Cells of the Nervous System: Function and Dysfunction (L. Hertz, ed.). Elsevier Science Publ., Amsterdam, The Netherlands. Adv. Mol. Cell Biol. 31, 461-475 (2004).
Sarup, A., Larsson, O.M. and Schousboe, A. Characterization of the substrate binding site in GABA transporters. In: Strategies in Molecular Neuropharmalogy (A. Schousboe and H. Bräuner-Osborne, eds.). Humana Press, Totowa, NJ, pp. 175-190 (2004).
Bak, L.K., Waagepetersen, H.S., Schousboe, A. Role of astrocytes in depolarization-coupled release of glutamate in cerebellar cultures. Neurochem. Res. 29, 257-265 (2004).
Poulsen, C.F., Simeone, T.A., Maar, T.E., Smith-Swintosky, V., White, H.S. and Schousboe, A. Modulation by topiramate of AMPA and kainate mediated calcium influx in cultured cerebral cortical, hippocampal and cerebellar neurons. Neurochem. Res. 29, 275-282 (2004).
Fiszman, M.L. and Schousboe, A. Role of calcium and kinases on the neurotrophic effect induced by γ-aminobutyric acid. J. Neurosci. Res. 76, 435-441 (2004).
Schousboe, A., Sarup, A., Bak, L.K., Waagepetersen, H.S. and Larsson, O.M. Role of astrocytic transport processes in glutamatergic and GABAergic neurotransmission. Neurochem. Int. 45, 512-527 (2004).
Sonnewald, U., Schousboe, A., Qu, H. and Waagepetersen, H.S. Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy. Neurochem. Int. 45, 305-310 (2004).
Jayakumar, A.R., Rama Rao, K.V., Schousboe, A. and Norenberg, M.D. Glutamine-induced free radical production in cultured astrocytes. Glia 46, 296-301 (2004).
Lund, T.M., Christensen, E., Kristensen, A.S., Schousboe, A. and Lund, A.M. On the neurotoxicity of glutaric, 3-hydroxy glutaric and transglutaconic acids in glutaric acidemia type 1. J. Neurosci. Res. 77, 143-147 (2004).
Sonnewald, U., Olstad, E., Qu, H., Babot, Z., Crist fol, R., Sunol, C., Schousboe, A. and Waagepetersen, H.S. First direct demonstration of extensive GABA synthesis in mouse cerebellar neuronal cultures. J. Neurochem. 91, 796-803 (2004).
Schousboe, A., Sarup, A., Larsson, O.M. and White, H.S. GABA transporters as drug targets for modulation of GABAergic activity. Biochem. Pharmacol. 68, 1557-1563 (2004).
Schousboe, A., Larsson, O.M., Sarup, A., and White, H.S. Role of the betaine/GABA transporter (BGT-1/GAT2) for the control of epilepsy. Eur. J. Pharmacol. 500, 281-287 (2004).
Zieminska, E., Hilgier, W., Waagepetersen, H.S., Hertz, L., Sonnewald, U., Schousboe, A. and Albrecht, J. Analysis of glutamine accumulation in rat brain mitochondria in the presence of a glutamine uptake inhibitor, histidine, reveals glutamine pools with a distinct access to deamidation. Neurochem. Res. 29, 2121-2123 (2004).
Sonnewald, U., Schousboe, A. and Waagepetersen, H.S. Neuronal-astrocytic interactions (TCA cycling). In: The Role of Glia in Neurotoxicity, 2. Ed. (M. Aschner and L.G. Costa, eds.). CRC Press, Florida, pp. 111-123 (2005).
Brown, A.M., Sickmann, H.M., Fosgerau, K., Lund, T.M., Schousboe, A., Waagepetersen, H.S. and Ransom, B.R. Astrocyte glycogen metabolism is required for neural activity during aglycaemia or intense stimulation in mouse white matter. J. Neurosci. Res. 79, 64-80 (2005).
Bak, L.K., Sickmann, H.M., Schousboe, A. and Waagepetersen, H.S. Activity of the lactate-alanine shuttle is independent of glutamate-glutamine cycle activity in cerebellar neuronal-astrocytic cultures. J. Neurosci. Res. 79, 88-96 (2005).
Waagepetersen, H.S., Melø, T., Schousboe, A. and Sonnewald, U. Homeostasis of neuroactive amino acids in cultured cerebellar and neocortical neurons is influenced by environmental cues. J. Neurosci. Res. 79, 97-105 (2005).
Jensen, M.L., Schousboe, A. and Ahring, P. Charge selectivity of the Cys-loop family of ligand gated ion channels. J. Neurochem. 92: 217-225 (2005).
Clausen, R.P., Moltzen, E.K., Perregaard, J., Lenz, S.M., Sanchez, C., Falch, E., Frølund, B, Sarup, A., Larsson, O.M, Schousboe, A. and Krogsgaard-Larsen, P. Selective inhibitors of GABA uptake: Synthesis and Molecular Pharmacology of 3-hydroxy-4-N-methylamino-4,5,6,7-tetrahydro-1,2-benzo[d]isoxazole analogues. Bioorg. Med. Chem. 13, 895-908 (2005).
White, H.S., Watson, W.P., Hansen, S., Slough, S., Sarup, A., Bolvig, T., Petersen, G., Larsson, O.M., Clausen, R.P., Frølund, B., Krogsgaard-Larsen, P. and Schousboe, A. First demonstration of a functional role for CNS betaine/GABA transporter (mGAT2) based on synergistic anticonvulsant action among inhibitors of mGAT1 and mGAT2. J. Pharmacol. Exp.Therap. 312: 866-874 (2005).
Jensen, M.L., Pedersen, L.N., Timmermann, D.B., Schousboe, A. and Ahring, P. Mutational studies using a cation-conducting GABAA receptor reveal the selectvity determinants of the Cys-loop family of ligand-gated ion channels. J. Neurochem. 92: 962-972 (2005).
Frandsen, A., Pickering, D.S., Vestergaard, B., Kasper, C., Nielsen, B.B., Greenwood, J.R., Campiani, G., Fattorusso, C., Gajhede, M., Schousboe, A. and Kastrup, J.S. Tyr702 is an important deeterminant of agonist binding and domain closure of the ligand-binding core of GluR2. Mol. Pharmacol. 67: 703-713 (2005).
Nielsen, B.B., Pickering, D.S., Greenwood, J.R., Brehm, L., Gajhede, M., Schousboe, A. and Kastrup, J.S. Exploring the GluR2 ligand-binding core in complex with the bicyclical AMPA analogue (S)-4-AHCP. FEBS J. 272: 1639-1648 (2005).
Dalskov, S.-M., Immerdal, L., Niels-Christiansen, L.-L., Hansen, G.H., Schousboe, A. and Danielsen, E.M. Lipid raft localization of GABAA receptor and Na+, K+-ATPase in discrete microdomain clusters in rat cerebellar granule cells. Neurochem. Int. 46: 489-499 (2005).
Waagepetersen, H.S., Qu, H., Sonnewald, U., Shimamoto, K. and Schousboe, A. Role of glutamine and neuronal glutamate uptake in glutamate homeostasis and synthesis during vesicular release in cultured glutamatergic neurons. Neurochem. Int. 47: 92-102 (2005).
Rogers, A., Schmuck, G., Scholz, G., Griffiths, R., Meredith, C., Schousboe, A., Campiani, G. and Williams, D.C. Improvements in an in-vitro assay for excitotoxicity by measurement of early gene (c-f mRNA) levels. Arch. Toxicol. 79: 129-139 (2005).
Olsen, M., Sarup, A., Larsson, O.M. and Schousboe, A. Effect of hyperosmotic conditions on the expression of the betaine-GABA-transporter (BGT-1) in cultured mouse astrocytes. Neurochem. Res. 30: 855-865 (2005).
Sickmann, H.M., Schousboe, A., Fosgerau, K. and Waagepetersen, H.S. Compartmentation of lactate originating from glycogen and glucose in cultured astrocytes. Neurochem. Res. 30, 1295-1304 (2005).
McKenna, M.C., Gruetter, R., Sonnewald, U., Waagepetersen, H.S. and Schousboe, A. Energy metabolism of the brain. In: Basic Neurochemistry: Molecular, Cellular, and Medical Aspects, 7th ed. (G.J. Siegel, B.W. Agranoff, R.W. Albers, S.K. Fisher and M.D. Uhler, eds.). Lippincott-Raven, Philadelphia, pp. 531-557 (2005).
Schousboe, A. and Waagepetersen, H.S. Role of astrocytes in glutamate homeostasis: Implications for excitotoxicity. Neurotoxicity Res. 8, 221-225 (2005).
Albrecht, J. and Schousboe, A. Taurine interaction with neurotransmitter receptors in the CNS: An update. Neurochem. Res. 30, 1615-1621 (2005).
