Sven Frøkjær

Scientific Career

Dean of Faculty, The Faculty of Pharmaceutical Sciences, University of Copenhagen, 2007
Rector at The Danish University of Pharmaceutical Sciences, 2003 
Professor at the Department of Pharmaceutics, the Royal Danish School of Pharmacy, 1993 

Novo Nordisk A/S:
Manager, Enzyme Supplementation Technology. Responsible for identification and development of products within the field of therapeutic enzyme applications.
Director, Nutritional Product R&D, FERROSAN. Responsible for implementing clinical nutrition in the FERROSAN organization. Continuously responsible for the R&D activities within clinical nutrition, including clinical documentation.
Manager, Product R&D, Food Ingredients Team. Responsible for development and production of products for clinical nutrition, including clinical documentation, 1985
Manager, Physical Chemistry. Responsible for preformulation activities and drug delivery systems for peptides, 1982
Manager, Pharmaceutical R&D II. Responsible for biopharmaceutics and drug delivery R&D, 1982
Responsible for R&D on drug delivery systems (liposomes), 1974

Assistant Professor at the Department of Pharmaceutics, the Royal Danish School of Pharmacy, 1974
PhD in Physical Chemistry, Department of Pharmaceutics, the Royal Danish School of Pharmacy, 1973 

Research Interests

Peptide and protein formulation with a special emphasis on particulate drug delivery systems, e.g. microspheres, liposomes and lipid emulsions, and peptide transport across biological membranes including carrier-mediated mechanisms (approx. 80 publications in these fields).

Publications

Publications 2007 to present
Press link to see list in CURIS

2003 to 2006
Stigsnæs, P; Frøkjær, S; Bjerregaard, S; van de Weert, M; Kingshott, P; Møller, E.H:
Characterisation and physical stability of PEGylated glucagons.
International Journal of Pharmaceutics (2007) (in press)
Foged C, Mørck Nielsen H, Frøkjær, S.
Liposomes for Phospholipase A2 Triggered siRNA Release: Preparation and in vitro Test
International Journal of Pharmaceutics, 331(2007)160-166
Donsmark, J.; Jorgensen, L.; Mollmann, S.; Frokjaer, S.; Rischel, C.
Kinetics of Insulin Adsorption at the Oil-Water Interface and Diffusion Properties of Adsorbed Layers Monitored Using Fluorescence Correlation Spectroscopy
Pharm Res 2006; 23(1): 148-155
Huus, K.; Havelund, S.; Olsen, H.B.; Sigurskjold, B.W.; van de Weert, M.; Frokjaer, S.
Ligand Binding and Thermostability of Different Allosteric States of the Insulin Zinc-Hexamer
Biochemistry 2006; 45: 4014-4024
Mollmann, S.H.; Bukrinsky, J.T.; Elofsson, U.; Elversson, J.; Frokjaer, S.; Thalberg, K.; Millqvist-Fureby, A.
The Stability of Insulin in Solid Formulations Containing Melezitose and Starch. Effects of Processing and Excipients
Drug Dev Ind Pharm 2006; 32:765-777
Mollmann, S.H.; Jogensen, L.; Bukrinsky, J.T.; Elofsson, U.; Norde, S.; Frokjaer, S.
Interfacial adsorption of insulin Conformational changes and reversibility of adsorption
Eur J Pharm Sci 2006; 27: 194-204
Møller, E.H.; Fano, M.; van de Weert, M.; Frokjaer, S.
Comparability of protein pharmaceuticals and the suitability of methods for secondary structure analysis
Pharm Tech Eur July 2006: 38-42
Vermehren, C.; Frokjaer, S.; Aurstad, T.; Hansen, J.
Lung surfactant as a drug delivery system
Int J Pharm 2006; 307: 89-92
Brønnum, H.; Seested, T.; Hellgren, L.I.; Brix, S.; Frøkiær, H.
Milk-Derived GM₃ and GD₃ Differentially Inhibit Dendritic Cell Maturation and Effector Functionalities
Scand J Immunol 2005; 61(6): 551-557
Foged, C.; Brodin, B.; Frokjaer, S.; Sundblad, A.
Particle size and surface charge affect particle uptake by human dendritic cells in an in vitro model.
Int J Pharm. 2005;298(2):315-22
Frokjaer, S.; Hovgaard, L.; van de Weert, M.
Formulation, Stability, and Characterization of Protein and Peptide Drugs
Book chapter in: Biomaterials for Delivery and Targeting of Proteins and Nucleic Acids, ed. by Ram I. Mahato, CRC Press, 2005
Frokjaer, S.; Otzen, D.E.
Protein drug stability: A formulation challenge
Nat Rev Drug Discov 2005; 4: 298-306
Huus, K.; Havelund, S.; Olsen, H.B.; van de Weert, M.; Frokjaer, S.
Thermal Dissociation and Unfolding of Insulin
Biochem 2005;44: 11171-11177
Mollmann, S.H.; Elofsson, U.; Bukrinsky, J.T.; Frokjaer, S.
Displacement of Adsorbed Insulin by Tween 80 Monitored Using Total Internal Reflection Flourescence and Ellipsometry
Pharm Res 2005; 22 (11): 1931-1941
Mollmann, S.H.; Bukrinsky, J.T.; Frokjaer, S.; Elofsson, U.
Adsorption of human insulin and Asp^B28 insulin on a PTFE-like surface
J Colloid Interface Sci 2005; 286: 28-35
Soenderkaer, S.; van de Weert, M.; Hansen, L.L.; Flink, J.; Frokjaer, S.
Evaluation of statistical design/modeling for prediction of the effect of amino acids on agitation-induced aggregation of human growth hormone and human insulin
J Drug Del Sci Tech (2005), 15 (6): 427-434
Bravo, S.A.; Nielsen, C.U.; Amstrup, J.; Frokjaer, S.; Brodin, B.
Epidermal growth factor decreases PEPT2 transport capacity and expression in the rat kidney proximal tubule cell line SKPT0193 cl. 2.
Am J Physiol Renal Physiol (2004) 286; F385-F393
Bravo, S.A.; Nielsen, C.U.; Amstrup, J.; Frokjaer, S.; Brodin, B.
In depth evaluation of Gly-Sar transport parameters as a function of culture time in the Caco-2 cell model.
Eur. J. Pharm. Sci. (2004) 21; 77-86
Jørgensen, L.; Kim, D.H.; Vermehren, C.; Bjerregaard, S.; Frokjaer, S.
Micropipette Manipulation: A Technique to Evaluate the Stability of Water-in-Oil Emulsions Containing Proteins
J. Pharm. Sci. (2004) 93; 2994-3003
Jørgensen, L.; van de Weert, M.; Vermehren, C.; Bjerregaard, S.; Frokjaer, S.
Probing structural changes in proteins incorporated into water-in-oil emulsions.
J. Pharm. Sci. (2004) 93; 1847-1859
Soenderkaer, S.; Carpenter, J.F.; van de Weert, M.; Hansen, L.L.; Flink, J.; Frokjaer, S.
Effects of sucrose on rFVIIa aggregation and methionine oxidation
Eur. J. Pharm. Sci. (2004); 21; 597-606
Steffansen, B.; Nielsen, C.U.; Brodin, B.; Eriksson, A.H.; Andersen, R.; Frokjaer, S.
Intestinal solute carriers: an overview of trends and strategies for improving oral drug absorption
Eur. J. Pharm. Sci. (2004) 21; 3-16
Vermehren, C.; Hansen H.S.; Clausen-Beck, B.; Frokjaer S.
In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes.
Int. J. Pharm. (2003) 254; 49-53
Uversky, V.N.; Garriques, L.N.; Millett, I.S.; Frokjaer, S.; Brange, J.; Doniach, S.; Fink, A.L.
Prediction of the association state of insulin using spectral parameters.
J. Pharm. Sci. (2003) 92; 847-58
Jørgensen, L.; Vermehren, C.; Bjerregaard, S.; Frokjaer, S.
Secondary Structure alteration in insulin and growth hormone water-in-oil emulsions.
Int. J. Pharm. (2003) 254; 7-10
Thomsen, A.E.; Friderichsen, G.M.; Sørensen, A.H.; Andersen, R.; Nielsen, C.U.; Brodin, B.; Begtrup, M.; Frokjaer, S.; Steffansen, B.
Prodrugs of purine and pyrimidine analogues for the intestinal di/tri-peptide transporter PepT1: affinity for hPepT1 in Caco-2cells, drug release in aqueous media and in vitro metabolism.
J. Control. Release (2003) 86; 279-292
Nielsen, C.U.; Amstrup, J.; Nielsen, R.; Steffansen, S.; Frokjaer, S.; Brodin, B.
Epidermal growth factor and insulin short-term increase hPepT1-mediated glycylsarcosine uptake in Caco-2 cells.
Acta Physiol. Scand. (2003) 178; 139-148
Steffansen, B., Thomsen, A.E. & Frokjaer, S.
Prodrugs, in Drug Availability.
Eds. H. van de Waterbeemd, H. Lennernäs & P. Artursson, Wiley-VCH, Weinheim (2003)